產(chǎn)品詳情
簡單介紹:
Mouseanti-HumanIgMAntibody(HRP)
詳情介紹:
Immunogen | Highly purified monoclonal IgM isolated from pooled Human serum |
Clone | NI 179 |
Isotype | IgG1 |
Characteristics | Molar Ratio: Peroxidase/IgG: aprox. 1.7 |
Purification | Immunoaffinity Chromatography |
Background | Synonyms: Human Immunoglobulin M |
Research Area | Secondary Antibodies, Immunology |
Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
Restrictions | For Research Use only |
Reconstitution | Restore by adding 0.5?mL sterile distilled water |
Concentration | 0.4 mg/mL |
Buffer | PBS,?pH 7.2 without foreign proteins, None as it may interfere with the antibody activity |
Preservative | Without preservative |
Handling Advice |
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody lyophilized at 2-8 °C for one month or (in aliquots) at -28 °C for longer. Diluted ascites should be stored at 2-8 °C, not refrozen, and preferably used the same day. |
Background publications |
Jefferis, Reimer, Skvaril, de Lange, Goodall, Bentley, Phillips, Vlug, Harada, Radl: "Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study." in: Immunology letters, Vol. 31, Issue 2, pp. 143-68, 1992 (PubMed).
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