產(chǎn)品詳情
簡單介紹:
Mouseanti-HumanIgGAntibody(AlkalinePhosphatase(AP))
詳情介紹:
Clone | H2 |
Isotype | IgG2b |
Background | IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen s. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75?% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66?%), IgG2 (23?%), IgG3 (7?%) and IgG4 (4?%).Synonyms: Human Immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
ELISA: 1/5000-1/10,000. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
Format | Liquid |
Buffer | 50?mM Tris/1?mM MgCl2/50?% Glycerol,?pH 8.0, containing 0.09?% Sodium Azide as preservative. |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice |
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed. |
Storage | 4 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C. |