產(chǎn)品詳情

  • 產(chǎn)品名稱：Rabbitanti-Mouse(Murine)IgGAntibody(FITC)

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  • 產(chǎn)品廠商：Cloud-Clone
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Rabbitanti-Mouse(Murine)IgGAntibody(FITC)
詳情介紹：
Immunogen The antibody is a rabbit polyclonal antibody raised against IgG conjugated to fitc.
Isotype IgG
Specificity It has been selected for its ability to recognize IgG in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Format Liquid
Concentration 200 μg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months
Product cited in: Wei, Zhang, Hong, Chen, Deng, He, Chen: "Icariin promotes osteogenic differentiation of rat bone marrow stromal cells by activating the ERα-Wnt/β-catenin signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 84, pp. 931-939, 2016 (PubMed).

Mei, Zou, Fu, Xia, Zhou, Zhang, Tuo, Wang, Jin: "Substance P activates the Wnt signal transduction pathway and enhances the differentiation of mouse preosteoblastic MC3T3-E1 cells." in: International journal of molecular sciences, Vol. 15, Issue 4, pp. 6224-40, 2014 (PubMed).

Fu, Mei, Wang, Zou, Liu, Pei, Bi, Jin: "Neuropeptide substance P improves osteoblastic and angiogenic differentiation capacity of bone marrow stem cells in vitro." in: BioMed research international, Vol. 2014, pp. 596023, 2014 (PubMed).