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Application Notes
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Can be used: As unlabelled primary or secondary antibody reagent for indirect detection of IgM in Mouse cells, tissues and body fluids in Immunofluorescence and Immunoenzyme assay methods. For the production of immunoconjugates with a selected marker. To prepare immunoaffinity adsorbents by coupling to an artificial carrier. In non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent. In Western blotting. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling techniques, the optimum concentration of this product should be established before. Recommneded Working Dilutions : Histochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. Precipitin titre not less than 1/64 when tested against normal Mouse serum in agar-block immunodiffusion titration. Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity.
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