產(chǎn)品詳情

  • 產(chǎn)品名稱：DUB-Detector

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：ActiveMotif
  • 產(chǎn)品文檔：
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DUB-Detector
詳情介紹：
Specificity The DUB-Detector? assay was used to screen for deubiquitination activity from the provided HeLa nuclear extracts at 0.313, 0.625 and 1.25 μg per reaction in the presence or absence of 1 μM Inhibitor for 20 minutes at room temperature.
Comment

Following the incubation, Fluorescent Substrate (100 nM) was added to each well and the fluorescent intensity was measured (ex 485 nm, em 535 nm) every two minutes. The data was plotted as a kinetic graph (Figure 1). Fluorescent intensity for the 1.25 μg reaction at a 60 minute kinetic reading should have 10-fold higher signal than the initial 0 minute kinetic reading. Lot 10510001 had a 22-fold increase in fluorescent intensity. There should be no significant increase in fluorescence for the wells treated with inhibitor.
Restrictions For Research Use only
Storage Comment The Fluorescent Substrate, Inhibitor and HeLa nuclear extract should be stored at -80C and protected from light. Assay Buffer AM2 and 1 M DTT can be stored at -20C. The 96-well half area plate, plate sealer and reagent reservoir can all be stored at room temperature. Please see the manual for complete details.