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Format
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Lyophilized
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Reconstitution
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For reconstitution add 1.1?mL of sterile water. Let it stand 30?minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
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Buffer
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10 mM Sodium Phosphate, 0.15 M SodiumChloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
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Preservative
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Kathon CG
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Handling Advice
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 mL of sterile water add 1.1 mL of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
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Storage
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-20 °C
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Storage Comment
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Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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