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  • 產(chǎn)品名稱：Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(AlkalinePhosphatase(AP))

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  • 產(chǎn)品廠商：Agrisera
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簡單介紹：
Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(AlkalinePhosphatase(AP))
詳情介紹：
Immunogen Purified Rabbit IgG
Isotype IgG
Predicted Reactivity rabbit IgG (H&L)
Characteristics Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)
Comment

This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Restrictions For Research Use only
Format Liquid
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Storage Comment Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Background publications Kay, Clark, Maurer, Ying: "Elevations in T-helper-2-initiating cytokines (interleukin-33, interleukin-25 and thymic stromal lymphopoietin) in lesional skin from chronic spontaneous ('idiopathic') urticaria." in: The British journal of dermatology, Vol. 172, Issue 5, pp. 1294-302, 2015 (PubMed).