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  • 產(chǎn)品名稱：Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)

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  • 產(chǎn)品廠商：Agrisera
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簡單介紹：
Donkeyanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)
詳情介紹：
Isotype IgG
Cross-Reactivity Sheep (Ovine), Chicken, Goat, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
Characteristics Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG.
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes Needs to be determined by end user for all immunoassay applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 1.1?mL of sterile water. Let it stand 30?minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Handling Advice Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Storage 4 °C
Storage Comment Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Background publications Petersen, Andersen: "Simultaneous isolation of mRNA and native protein from minute samples of cells." in: BioTechniques, Vol. 56, Issue 5, pp. 229-37, 2014 (PubMed).