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  • 產(chǎn)品名稱:Mouseanti-HumanIgG1,IgG2,IgG2Fc,IgG3,IgG4Antibody

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  • 產(chǎn)品廠商:BioLeaf
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簡單介紹:
Mouseanti-HumanIgG1,IgG2,IgG2Fc,IgG3,IgG4Antibody
詳情介紹:
Immunogen Highly purified monoclonal IgG subclasses isolated from pooled human serum.
Specificity The antibodies do not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species.
Characteristics The reactivity of the antibody is restricted to a subclass specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.

Monoclonal antibody concentration in the solution is 1.0 mg/mL. No foreign proteins added.
Purification Ascites
Background To identify the presence of IgG subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Application Notes The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1,000 upwards, in Western blotting 1:2,000 to 1:10,000. These data should be interpreted as general recommendations only.
Comment

Physical form: Delipidated, heat inactivated, lyophilized stable ascites.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).
Handling Advice Avoid repeated thawing and freezing.
If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product.
Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Shelf life:
Lyophilized at +4 °C: at least 10 years.
Reconstituted at or below -20 °C: 3-5 years.
Reconstituted at +4 °C: 7 days

Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C
Storage Comment The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C.
Expiry Date 36 months