產(chǎn)品詳情

  • 產(chǎn)品名稱：Rabbitanti-HumanIg(Chainlambda),(LightChain)Antibody

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  • 產(chǎn)品廠商：SpringBio
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Rabbitanti-HumanIg(Chainlambda),(LightChain)Antibody
詳情介紹：
Immunogen Free lambda light chain from pooled human IgG lambda myeloma sera.
Clone SP147
Isotype IgG
Characteristics Cellular Localization: Cytoplasm
Purification Purified by Protein A/G
Alternative Name Immunoglobulin Ig
Background Lambda light chains are polypeptide chains located in the cell membrane and cytoplasmic regions of normal B cells and plasma cells. The combination of lambda light chains and heavy chains forms immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains. Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell will only contain a single light chain class. This clonal restriction may be used to indicate the polyclonal or monoclonal nature of B cell and plasma cell populations. The ratio of Kappa to Lambda is about 2:1. The level of kappa or lambda can be greatly elevated in multiple myeloma or other B cell malignancies.
Molecular Weight 25 kDa
Application Notes IHC Dilution: 1:100
Comment

Positive Control:
IHC (p): Tonsil
WB: Human spleen lysate
Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (ABIN1687988) Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (ABIN1687988) Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (Image 2)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (ABIN1687988) Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (Image 3)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (ABIN1687988) Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (Image 4)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (ABIN1687988) Rabbit anti-Human Ig (Chain lambda), (Light Chain) antibody (Image 5)