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Format
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Lyophilized
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Reconstitution
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For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 μg of primary antibody with 5 μg of Fab fragments) in a total volume of 10 μL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image
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Buffer
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PBS
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Handling Advice
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Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
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Storage
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-20 °C
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Storage Comment
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Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.
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