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  • 產(chǎn)品名稱：Mouseanti-HumanIgG2Antibody

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Mouseanti-HumanIgG2Antibody
詳情介紹：
Immunogen Native Protein IgG2There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, IgG2-B, and IgG2-A/B, which differ by the disulfide connectivity at the hinge region. IgG2, the second largest part of IgG isotypes in humans, comprises 20-25 % of the main subclass and is the prevalent immune response against carbohydrate-/polysaccharid antigens.
The antibody is a mouse monoclonal antibody raised against IgG2.
Isotype IgG
Specificity It has been selected for its ability to recognize IgG2 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Research Area Secondary Antibodies
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions For Research Use only
Format Liquid
Concentration 500 μg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months